Recombinant Human IFN-gamma

Molecule:

IFN-gamma

Synonyms:

IFN-gamma, Immune interferon

Species:

Human

Cat. No.:

CRI000C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,020.00 / Special Price: $840.00 BUY

Description:

Interferon-gamma (IFN-gamma), also known as Type II interferon or immune interferon, is a cytokine produced primarily by lymphocytes and natural killer cells. The protein shares no significant homology with IFN-beta or the various IFN-alpha family proteins. Mature IFN-gamma exists as noncovalently-linked homodimers. Human IFN-gamma is highly species specific and is biologically active only in human and primate cells. IFNgamma was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-gamma induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-gamma also augments TH1 cell expansion and may be required for TH1 cell differentiation.

Entrez Symbol:

IFNG

Uniprot Name:

Interferon gamma

Uniprot ID:

P01579

Data PDF:

CRI000

Source:

E. coli

Molecular Weight:

16.9 kDa (144 aa) monomer

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered solution in 10 mM sodium phosphate, 50 mM NaCl, pH 7.5

Purity:

≥ 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

Demonstrates dose-dependent inhibition of cytopathic effect using EMC-infected A549 cells.

Specific Activity:

≥ 1.0 x 10e7 Units/mg

Endotoxin Level:

≤ 1 EU/µg of protein by kinetic LAL analysis (50% confidence range).

Amino Acid Sequence:

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFK N FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVM AE LSPAAKTGKR KRSQMLFQGR RASQ

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.