Insulin-like growth factor I IGF-1, Mechano growth factor, MGF, Somatomedin-C

Human

AQU500 pdf (datasheet)

0.5 mg

$1,860.00 BUY

Biotinyl human insulin-like growth factor-I (biotinyl IGF-I) is made by reacting human IGFI with Sulfo-NHS-Biotin reagent to label the primary amines of the IGF-I molecule. A mixture of biotinylated forms are produced, the major species being di-biotinyl IGF-I. Biotinyl IGF-I has been prepared with a spacer to minimize steric hindrance during subsequent avidin binding.

IGF1

P05019

AQU050

E. coli

8327 Da, di-biotinyl, predominant species

7988 Da mono-biotinyl

8683 Da tri-biotinyl

Lyophilized from Acetonitrile, 0.1% TFA, sealed under dry nitrogen at a slight vacuum (~25 kPa)

High purity, receptor grade IGF-I (> 95% by HPLC) was used as the source material for biotinylation.

Store as supplied for up to 1 year at 2-8°C.

Protocol 3004 Procedure for Western Ligand blotting using biotinylated IGF-I or IGF-II