Human Recombinant IGF-I [Leu24], Receptor Grade

Molecule:

IGF-I

Synonyms:

Insulin-like growth factor I, Somatomedin-C, Mechano growth factor, MGF

Species:

Human

Cat. No.:

ZU020 pdf (datasheet)

Quantity/Size:

20 μg

Price:

$250.00 BUY

Description:

Human [Leu24] insulin-like Growth Factor-I ([Leu24] IGF-I) is an analog of human IGF-I which has a Leucine substitution in place of Tyrosine at position 24 of the human IGF-I sequence. [Leu24]IGF-I has normal binding to acid-stripped human serum binding proteins but has reduced binding to the type 1 IGF receptor and to the insulin receptor compared to IGF-I. It is a useful analogue for studying the relative effects of IGF-I through the type 1 receptor and IGF binding proteins.

Entrez Symbol:

IGF1

Uniprot Name:

Insulin-like growth factor I

Uniprot ID:

P05019

Data PDF:

ZU020

Source:

E. coli

Molecular Weight:

7599 Da

Physical State:

Lyophilized

Purity:

>95% by HPLC analysis

Biological Activity:

Stimulation of protein synthesis in rat L6 myoblasts: ED50 < 100 ng/ml
IGF type 1 receptor binding assay (ED50 > 100 ng/ml)
IGF binding protein assay: ED50 < 10 ng/ml)

Endotoxin Level:

< 0.1 EU/µg.

Amino Acid Sequence:

N-terminal 5 residues confirmed

Reconstitution:

See Protocol 1000, Handling of IGF-I, IGF-II and IGF analogs.

Storage/Stability:

Stable as supplied for at least 2 years at 2-8°C