Synonyms:
Somatomedin A, GRDF
Description:
Insulin-like Growth Factor II (IGF2) is a major growth hormone made by Theca cells
during gestation. While IGF2 is known to engage the IGF1 receptor (IGF1R) to mediate
embryonic growth, IGF2 is also known to bind the IGF2 receptor (IGF2R).
Recombinant human IGF2 is a non-glycosylated protein, containing 67 amino acids.
Uniprot Name:
Insulin-like growth factor II
Molecular Weight:
Monomer, 7.5 kDa (67 aa)
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile filtered solution containing 0.1% Trifluoroacetic Acid (TFA).
Purity:
≥95% by reducing and non-reducing SDS-PAGE.
Biological Activity:
The ED50, calculated by the dose-dependent proliferation of FDC-P1 cells, is ≤15 ng/mL
Specific Activity:
≥ 6.7 X 104 U/mg
Endotoxin Level:
≤ 1 EU/µg
Amino Acid Sequence:
AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE
Reconstitution:
Centrifuge vial before opening. Reconstitute with sterile water at a concentration of 0.1 mg/mL, gently pipet up and down the sides of the vial to ensure full recovery of the protein into solution.
Storage/Stability:
Upon receipt, store at -20°C to -80°C for up to one year. Upon reconstitution as directed, the preparation is stable for up to 1 month at 2-8°C, 3 months at -20°C to -80°C. Long term storage reconstitute in working aliquots containing 0.1% BSA and store at -80°C. Avoid repeated freeze-thaw cycles
