Synonyms:
Somatamedin A, IGF-II, IGF2, IGF-2
Description:
Recombinant Human IGF-2 is non-glycosylated protein containing 67 amino acids and having a molecular mass of 7.5 kDa. Background: Insulin like Growth Factor 2, IGF-2, is a major growth hormone made by Theca cells during gestation. While IGF-2 is known to engage the IGF-1 receptor (IGF1R) to mediate embryonic growth, IGF-2 is also known to bind the IGF-2 receptor (IGF2R), which is thought to be signaling dead receptor that acts as a sink by binding up free IGF-2.
Uniprot Name:
Insulin-like growth factor II
Molecular Weight:
7.5 kDa, monomer
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Purity:
≥95% by Reducing and Non-Reducing SDS PAGE
Biological Activity:
The activity is determined by the dose dependent proliferation of FDC-P1 cells. The ED50 is ≤15 ng/mL (typical observed range 1-10 ng/mL)
Specific Activity:
≥ 6.7 x 104 units/mg
Endotoxin Level:
Less than ≤1 EU/µg as determined by Kinetic LAL (50% confidence range)
Amino Acid Sequence:
AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE
Reconstitution:
Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Storage/Stability:
Lyophilized recombinant human IGF-II, although stable at room temperature, should be stored desiccated at -20°C. Upon reconstitution, recombinant human IGF-II should be stored in working aliquots at -20°C. For long term storage, it is recommended that a carrier protein (0.1% HSA or BSA) should be added to the solution. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.
