Recombinant Human IGF-II Des(1-6) Receptor Grade

Molecule:

IGF-II

Synonyms:

Insulin-like growth factor II

Species:

Human

Cat. No.:

MU100 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$290.00 BUY

Description:

Human Des [1-6] insulin-like Growth Factor-II (IGF-II) is a 61 amino acid analogue of human IGF-II lacking the N-terminal hexapeptide Ala-Tyr-Arg-Pro-Ser-Glu. Human Des [1-6] IGF-II is more potent than IGF-II in vitro and in vivo. This increased potency is due to reduced binding of human Des [1-6] IGF-II to most of the IGF binding proteins which modify the biological actions of IGF-II. Human Des [1-6] IGF-II binds to both the type 1 and type 2 IGF receptors with similar affinity to wild type IGF-II. This is a high quality research reagent for use in studies on cell growth, IGF receptors and IGF binding proteins.

Entrez Symbol:

IGF2

Uniprot Name:

Insulin-like growth factor II

Uniprot ID:

P01344

Data PDF:

MU020

Source:

E. coli

Molecular Weight:

6.765 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from 0.1 M acetic acid under dry nitrogen at a slight vacuum (-25 kPa)

Purity:

>95% by HPLC

Biological Activity:

Stimulation of protein synthesis in rat L6 myoblasts: ED50 <75 ng/ml
IGF type 1 receptor binding assay: ED50 < 50 ng/ml
IGF type 2 receptor binding assay: ED50 < 30 ng/ml
IGF binding protein assay: ED50 > 1000 ng/ml

Endotoxin Level:

< 0.1 EU/µg

Reconstitution:

See Protocol #1000 included.

Storage/Stability:

Store in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.