Synonyms:
Growth hormone-dependent binding protein, IBP3, BP-53
Description:
Recombinant Human IFGBP3 is a single non-glycosylated polypeptide chain containing 264 amino acids.
Background: Insulin-like Growth Factor-Binding Protein 3(IGF-BP3) is a 30 kDa cysteine-rich secreted protein. It is the major IGF binding protein present in the plasma of human and animals and it is also found in α-granules of platelets. In addition to its ability to modulate the activity of IGF-I and IGF-II, IGF-BP3 exerts inhibitory effects on follicle stimulating hormone (FSH) activity. Decreased plasma levels of IGF-BP3 often results in dwarfism, whereas elevated levels of IGF-BP3 may lead to acromegaly. The expression of IGF-BP3 in fibroblasts is stimulated by mitogenic growth factors such as Bombesin, Vasopressin, PDGF, and EGF.
Molecular Weight:
~28.8 kDa
Formulation:
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Purity:
>98% by SDS-PAGE and HPLC analyses.
Biological Activity:
Fully biologically active when compared to standard. The ED50 as determined by inhibiting IGF-II induced proliferation of serum free human MCF-7 cells is less than 200 ng/ml, corresponding to a specific activity of ＞ 5.0 × 103 IU/mg in the presence of 15 ng/ml of rHuIGF-II.
Endotoxin Level:
Less than 1EU/μg of rHuIGF-BP3 as determined by LAL method.
Amino Acid Sequence:
GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Stable at 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, store in working aliquots at -20°C-80°C. Avoid repeated freeze/thaw cycles.
