Recombinant Human IL-1 alpha

Molecule:

IL-1 alpha

Synonyms:

IL1A, IL1F1, IL-1 alpha

Species:

Human

Cat. No.:

CRI132C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,520.00 / Special Price: $2,720.00 BUY

Description:

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1 alpha and IL-1 beta have overlapping proinflammatory activities, to control fever induction, initiate rheumatoid arthritis and promote septic shock.

Entrez Symbol:

IL1A

Uniprot ID:

P01583

Data PDF:

CRI132

Source:

E. coli

Molecular Weight:

predicted 18.0 kDa, monomer (159 aa)

Physical State:

Lyophilized

Formulation:

Sterile filtered solution of 10 mM sodium phosphate, pH 7.5

Purity:

>95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 50 pg/ml, determined by a proliferation assay using murine D10S cells.

Specific Activity:

≤ 2.0 × 10e7 units/mg

Endotoxin Level:

<1 EU/µg, by kinetic LAL

Amino Acid Sequence:

SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store at -20°C to -80°C 1 year.