Recombinant Human IL-10

Molecule:

IL-10

Synonyms:

CSIF, TGIF, GVHDS, IL-10, IL10A

Species:

Human

Cat. No.:

CRI153A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$85.00 BUY

Description:

Interleukin-10 (IL-10) is a cytokine produced primarily by macrophages and type 2 T helper (Th2) cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. The IL-10 dimer binds to two IL10-R-alpha/ IL-10R1 chains, resulting in recruitment of two IL-10 R-beta/ IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3.

Entrez Symbol:

IL10

Uniprot Name:

Interleukin-10

Uniprot ID:

P22301

Data PDF:

CRI153

Source:

E. coli

Molecular Weight:

Noncovalent homodimer, 18.8 kDa (161 aa)

Physical State:

Lyophilized

Formulation:

Sterile filtered solution of 10 mM sodium phosphate, pH 7.5

Purity:

> 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 5 ng/ml, determined by proliferation of mouse MC/9 cells.

Specific Activity:

≤ 2.0 x 10e5 units/mg, calibrated against recombinant human IL-10 WHO International Standard (NIBSC code: 93/722).

Endotoxin Level:

< 1 EU/µg, by kinetic LAL

Amino Acid Sequence:

MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store as supplied at -20 °C to -80 °C for up to one year.