Synonyms:
AGIF (Adipogenesis Inhibitory Factor), oprelvekin, IL-11
Description:
Interleukin-11 (IL-11) is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T1165. IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF). The human IL-11 cDNA encodes a 199 amino acid residue precursor polypeptide with a 21 amino acid residue hydrophobic signal that is processed proteolytically to generate the 178 amino acid residue mature protein. IL-11 contains no cysteine residues or potential glycosylation sites.IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cell.
Molecular Weight:
19.3 kDa (179 aa)
Physical State:
Lyophilized
Formulation:
Lyophilized from a 0.2μm filtered aqueous solution containing 0.1% Trifluoroacetic acid (TFA).
Purity:
>95%, by reducing and non-reducing SDS-PAGE.
Biological Activity:
ED50 ≤ 10 ng/ml, determined by a cell proliferation assay using TF-1 cells. ED50 ≤ 2.5 ng/ml, determined by a cell proliferation assay using T11 cells.
Specific Activity:
> 1.0 × 10e6 IU/mg, by proliferation assay using TF-1 cells. > 4.0 × 10e6 IU/mg, by proliferation assay using T11 cells.
Endotoxin Level:
< 1EU/μg, by kinetic LAL.
Amino Acid Sequence:
MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Store at -20°C to -80°C for up to 1 year.
