Recombinant Human IL-11 Animal Free

Molecule:

IL-11

Synonyms:

AGIF (Adipogenesis Inhibitory Factor), oprelvekin, IL-11

Species:

Human

Cat. No.:

CRI176C-AF pdf (datasheet)

Quantity/Size:

1 mg

Price:

$5,300.00 / Special Price: $4,100.00 BUY

Description:

Interleukin-11 (IL-11) is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T1165. IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF). The human IL-11 cDNA encodes a 199 amino acid residue precursor polypeptide with a 21 amino acid residue hydrophobic signal that is processed proteolytically to generate the 178 amino acid residue mature protein. IL-11 contains no cysteine residues or potential glycosylation sites.IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cell.

Manufactured without Animal-derived products, in an Animal Free facility.

Entrez Symbol:

IL11

Uniprot ID:

P20809

Data PDF:

CRI176-AF

Source:

E. coli

Molecular Weight:

19.3 kDa (179 aa)

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2μm filtered aqueous solution containing 0.1% Trifluoroacetic acid (TFA).

Purity:

>95%, by reducing and non-reducing SDS-PAGE.

Biological Activity:

ED50 ≤ 10 ng/ml, determined by a cell proliferation assay using TF-1 cells. ED50 ≤ 2.5 ng/ml, determined by a cell proliferation assay using T11 cells.

Specific Activity:

> 1.0 × 10e6 IU/mg, by proliferation assay using TF-1 cells. > 4.0 × 10e6 IU/mg, by proliferation assay using T11 cells.

Endotoxin Level:

< 1EU/μg, by kinetic LAL.

Amino Acid Sequence:

MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL

Storage/Stability:

Store at -20°C to -80°C for up to 1 year.