Recombinant Human IL-12 p70

Molecule:

IL-12

Synonyms:

NKSF, CLMF, natural killer cell stimulating factor, cytotoxic lymphocyte maturation factor

Species:

Human

Cat. No.:

CS529B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$300.00 BUY

Description:

Recombinant Human IL-12 is a glycosylated heterodimeric polypeptide containing 503 amino acids, 197 amino acid residues of the p35 subunit and 306 amino acid residues of the p40 subunit that are linked by disulfide-bonds between cysteines. Interleukin-12 (IL-12), also known as NKSF or CLMF, is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

Entrez Symbol:

IL12A / IL12B

Data PDF:

CS529

Source:

HEK293 cells

Molecular Weight:

57 kDa

Formulation:

The IL-12 was lyophilized from 1 mg/ml in 1 x PBS

Purity:

>95% by SDS-PAGE analysis.

Biological Activity:

The specific activity was determined by the dose-dependent release of IFN-gamma from human NK92 cells in the presence of 20 ng/ml rIL-2 and is typically 0.1-0.5 ng/ml.

Amino Acid Sequence:

See datasheet

Storage/Stability:

Store lyophilized protein at -20°C to -80°C