Synonyms:
CTLA-8, Interleukin-17
Description:
IL-17A is a pro-inflammatory cytokine and a member of the IL-17 family of cytokines. It is produced by activated T cells and acts through its receptor, IL-17R. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-17A can stimulate the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Uniprot Name:
Interleukin-17A
Molecular Weight:
Dimer, 15.7/31.3 kDa (137/274 aa)
Formulation:
Lyophilized from a sterile filtered solution containing 0.1% Trifluoroacetic Acid (TFA).
Purity:
≥95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 < 10 ng/ml, as determined by dose-dependent production of IL-6 by mouse 3T3 cells.
Specific Activity:
≥ 1.0 x 105 U/mg
Endotoxin Level:
≤ 1 EU/µg, by kinetic LAL
Amino Acid Sequence:
MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVA
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL. Suspend product by gently pipetting solution down sides of vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Upon receipt, store at -20 °C to -80 °C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8 °C. For long term, store working aliquots at -20 °C to -80 °C for up to 3 months. For maximal stability, dilute to working aliquots in a 0.1% BSA solution. Avoid repeated freeze-thaw cycles.
