Recombinant Human IL-2

Molecule:

IL-2

Synonyms:

IL-2, T-cell Growth Factor, TCGF, Aldesleukin, Lymphokine

Species:

Human

Cat. No.:

CRI100C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$670.00 BUY

Description:

Interleukin-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. The receptor for IL-2 contains three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes.

Entrez Symbol:

IL2

Uniprot Name:

Interleukin-2

Uniprot ID:

P60568

Data PDF:

CRI100

Source:

E. coli

Molecular Weight:

15.5 kDa (134 aa) monomer

Physical State:

Lyophilized

Formulation:

Lyophilized from sterile filtered solution containing 0.1% Trifluoroacetic Acid (TFA)

Purity:

≥95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 3 ng/ml, determined by the dose-dependant proliferation of mouse CTLL-2 cells. In addition, this product has been tested for primary human T cell proliferation, with a typical ED50 < 1 ng/ml.

Specific Activity:

≥ 3.3 x 105 U/mg by CTLL-2 assay, The specific activity of this product calibrated against the recombinant human IL-2 WHO International Standard (NIBSC code: 86/500) is approximately 1.8 x 104 IU/μg.

Endotoxin Level:

≤1 EU/µg by kinetic LAL

Amino Acid Sequence:

MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSII STLT

Reconstitution:

Centrifuge vial prior to opening. Add sterile 10 mM HCl to a concentration of 0.1 mg/mL. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.