Recombinant Human IL-21

Molecule:

IL-21

Synonyms:

Interleukin-21, Za11, IL-21

Species:

Human

Cat. No.:

CRI172A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$130.00 BUY

Description:

Recombinant Human IL-21 is a single, non-glycosylated polypeptide chain containing 132 amino acids.

Background: Interleukin 21 (IL-21) is a member of the common-gamma chain family of cytokines with immunoregulatory activity. IL-21 plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer (NK) cells, B cells and cytotoxic T cells. IL-21 is produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (γc) found in other cytokine receptors. The IL-21R activates the JAK/STAT signaling pathway and is expressed on T, B, and NK cells. Within the B cell lineage, IL-21 is a switch factor regulating IgG1 and IgG3 antibody production. IL-21 also cooperates with IL-4 for the production of multiple antibody classes in B cells.

Entrez Symbol:

IL21

Uniprot Name:

Interleukin-21

Uniprot ID:

Q9HBE4

Source:

E. coli

Molecular Weight:

15.4 kDa (132 aa)

Physical State:

Lyophilized

Formulation:

Sterile-filtered solution with 10 mM sodium phosphate, pH 7.5.

Purity:

>95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 50 ng/ml, determined by dose-dependent proliferation of B9 cells. ED50 < 2.0 ng/ml, determined by dose-dependent proliferation of ANBL-6 cells.

Specific Activity:

>2.0 × 10e4 U/mg, determined by dose-dependent proliferation of B9 cells. >5.0 × 10e5 U/mg, determined by dose-dependent proliferation of ANBL-6 cells.

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store at -20°C to -80°C for up to 1 year.