IL-21

IL-21, CVID11, Za11

Human

CRI172C-AF pdf (datasheet)

1 mg

$5,300.00 / Special Price: $4,320.00 BUY

Interleukin 21 (IL-21) is a member of the common-gamma chain family of cytokines with immunoregulatory activity. IL-21 plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer (NK) cells, B cells and cytotoxic T cells. IL-21 is produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (γc) found in other cytokine receptors. The IL-21R activates the JAK/STAT signaling pathway and is expressed on T, B, and NK cells. Within the B cell lineage, IL-21 is a switch factor regulating IgG1 and IgG3 antibody production. IL-21 also cooperates with IL-4 for the production of multiple antibody classes in B cells.

Made without animal-derived components in an animal-free facility.

IL-21

Interleukin-21

Q9HBE4

CRI172-AF

E. coli

15.4 kDa (132 aa)

Lyophilized

Lyophilized from sterile-filtered 10 mM sodium phosphate, pH 7.5.

>95% by reducing and non-reducing SDS-PAGE

ED₅₀ < 50 ng/ml, determined by dose-dependent proliferation of B9 cells.
ED₅₀ < 2.0 ng/ml, determined by dose-dependent proliferation of ANBL-6 cells.

＞ 2.0 × 10⁴ U/mg, determined by dose-dependent proliferation of B9 cells.
＞ 5.0 × 10⁵ U/mg, determined by dose-dependent proliferation of ANBL-6 cells.

≤1 EU/µg of protein by kinetic LAL analysis

MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store at -20°C to -80°C for up to 1 year. Upon reconstitution, the preparation is stable for up to 1 month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C. For maximal stability, dilute to working aliquots in a 0.1% BSA solution. Avoid repeated freeze-thaw cycles.