Recombinant Human IL-2 Receptor subunit alpha, soluble

Molecule:

IL-2RA

Synonyms:

Interleukin-2 receptor subunit alpha, CD25

Species:

Human

Cat. No.:

CRI192B pdf (datasheet)

Quantity/Size:

25 μg

Price:

$520.00 BUY

Description:

The IL-2 receptor system consists of three non-covalently linked subunits termed IL-2Rα, IL-2Rß, and IL-2Rγ. The IL-2Rα is a type I transmembrane protein consisting of a 219 amino acid extracellular domain, a 19 amino acid transmembrane domain and a 13 amino acid intracellular domain, which is not involved in the transduction of IL-2 signals. Proteolytic processing of IL-2Rα releases the entire extracellular domain of IL-2Rα thereby generating a 219 amino acid soluble protein called soluble IL-2Rα (sIL-2Rα). The homodimeric form binds IL-2 (KD = 10 nM) and facilitates IL-2 signaling. The secreted sIL-2Rα is expressed on leukemia cells, lymphoma cells, newly activated T and B-cells, as well as on approximately 10% of NK cells.

Entrez Symbol:

IL2RA

Uniprot Name:

Interleukin-2 receptor subunit alpha

Uniprot ID:

P01589

Data PDF:

CRI192

Source:

CHO cells

Molecular Weight:

24.8 kDa (219 aa) predicted 31 kDa, apparent, due to glycosylation

Formulation:

Lyophilized from PBS

Purity:

> 98% by SDS-PAGE and HPLC analyses

Biological Activity:

ED50 typically 0.5 - 1.5 μg/ml, determined by its ability to increase the proliferation effect of IL-2 in mouse CTLL-2 cells, in the presence of 1 ng/ml of recombinant IL-2.

Amino Acid Sequence:

ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQ

Storage/Stability:

Stable at -20°C to -80°C for 1 year.