Synonyms:
IL-3, MCGF, MULTI-CSF, mast cell growth factor, mast-cell growth factor, P-cell stimulating factor, P-cell-stimulating factor, hematopoietic growth factor, multilineage-colony-stimulating factor, multipotential colony-stimulating factor
Description:
Interleukin-3 (IL-3) is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. IL-3 is able to induce the differentiation of hematopoietic stem cells to precursor cells of myeloid lineage (erythrocytes, megakaryocytes, granulocytes, dendritic cells and monocytes). It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. IL-3 is produced by activated T cells, mast cells.
Recombinant human IL-3 is a single, non-glycosylated polypeptide chain containing 134 amino acids.
Uniprot Name:
Interleukin-3
Molecular Weight:
15.2 kDa
Formulation:
Lyophilized from a sterile-filtered solution containing 0.1% Trifluoroacetic Acid (TFA).
Purity:
> 95 % by SDS-PAGE
Biological Activity:
Fully biologically active when compared to standard. The ED50, as determined by a proliferation assay using human TF-1 cells, is typically < 0.25 ng/ml.
Endotoxin Level:
≤1 EU/µg of protein by kinetic LAL analysis.
Amino Acid Sequence:
MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF
Reconstitution:
Centrifuge vial prior to opening. Add sterile 10 mM acetic acid to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.
