Recombinant Human IL-33

Molecule:

IL-33

Synonyms:

interleukin-33, IL-33, IL-1F11, DVS27-related protein, interleukin-1 family member 11, nuclear factor from high endothelial venules

Species:

Human

Cat. No.:

CRI225C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$5,680.00 / Special Price: $3,720.00 BUY

Description:

IL-33, encoded by IL-33 gene located on the Chr.9 in humans, is a 30 kDa proinflammatory protein belonging to the IL-1 superfamily and it shares less than 20% a.a. sequence identity with other members. IL-33 secreted by high endothelial venules at high levels, which is found in tonsils, peyer patches and mesenteric lymph nodes, but not in placenta. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation. It elicits its biological effects by interacting with IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 mature protein has 52-58% a.a. sequence identity with mouse and rat IL-33.

Entrez Symbol:

IL33

Uniprot ID:

O95760

Source:

E. coli

Molecular Weight:

Monomer, 18.1 kDa (160 aa)

Physical State:

Lyophilized

Formulation:

Sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Purity:

> 95.0% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 500 pg/ml, determined by a cell proliferation assay using mouse 10S cells.

Specific Activity:

≤2.0 × 10e6 U/mg

Endotoxin Level:

<1 EU/μg by kinetic LAL method.

Amino Acid Sequence:

MSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store at -20°C to -80° for up to 1 year.