Synonyms:
IL-4, BCGF, BCDF, BSF-1, HCGF, MCGF-2, MFF, TCGF-2
Description:
Interleukin-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy.
Recombinant Human IL-4 is a single, non-glycosylated polypeptide chain containing 130 amino acids.
Uniprot Name:
Interleukin-4
Molecular Weight:
Monomer, 15.1 kDa (130 aa)
Physical State:
Lyophilized
Formulation:
Sterile filtered solution containing 0.1% Trifluoroacetic Acid (TFA).
Purity:
≥95% by reducing and nonreducing SDS-PAGE
Biological Activity:
ED50 < 250 pg/ml, determined by cell proliferation of human TF-1 cells.
Specific Activity:
≥4.0 x 10e6 IU/mg, calibrated against rhIL-4 WHO International Standard (NIBSC code: 88/656).
Endotoxin Level:
≤1 EU/µg, kinetic LAL
Amino Acid Sequence:
MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Upon receipt, store at -20°C to -80°C for up to 1 year.
