Synonyms:
interleukin-4, BSF-1, binetrakin, pitrakinra, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, lymphocyte stimulatory factor 1
Description:
Recombinant Human IL-4 is a single, non-glycosylated polypeptide chain containing 130 amino acids.
Background: Interleukin-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy.
Uniprot Name:
Interleukin-4
Molecular Weight:
~15.1 kDa
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).
Biological Activity:
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells, < 0.25 ng/ml.
Specific Activity:
>4.0 x 10e6 U/mg
Endotoxin Level:
≤1 U/µg of protein by kinetic LAL analysis
Amino Acid Sequence:
MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Lyophilized protein is stable at room temperature for shipping purposes. Store desiccated at -20°C on receipt. After reconstitution, stable for ≤1 month at 2-8°C. For long term, store aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
