Synonyms:
BSF2, HGF, HSF, IFNB2
Description:
Recombinant Human IL-6 is a single, non-glycosylated polypeptide chain containing 185 amino acids.
Background:Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate an immune response. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells and is involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. In addition, IL-6 has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal transducing component gp130 (CD130).
Uniprot Name:
Interleukin-6
Molecular Weight:
21.0 kDa
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).
Purity:
>95.0% by SDS-PAGE
Biological Activity:
Fully biologically active when compared to standard. The biological activity as determined by proliferation of mouse 7TD1 cells, is < 0.25 ng/ml.
Specific Activity:
4 x 10e6 units/mg
Endotoxin Level:
≤1 U/µg of protein by kinetic LAL analysis
Amino Acid Sequence:
MPVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ DMTTHLILRS FKEFLQSSLR ALRQM
Reconstitution:
Centrifuge vial prior to opening. Add sterile 10 mM HCl to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Lyophilized protein is stable at room temperature for shipping purposes. Store desiccated at -20°C on receipt. After reconstitution, stable for ≤1 month at 2-8°C. For long term, store aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
