Synonyms:
IL-6, BSF2, HGF, HSF, IFNB2, B-cell differentiation factor
Description:
Interleukin-6 (IL-6) acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages and plays an essential role in the final differentiation of B-cells into Ig-secreting cells and is involved in lymphocyte and monocyte differentiation. In addition, IL-6 has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. IL-6 is produced at sites of acute and chronic inflammation, where it is secreted and signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal transducing component gp130 (CD130.
Uniprot Name:
Interleukin-6
Molecular Weight:
Monomer, 21 kDa (185 aa)
Physical State:
Lyophilized
Formulation:
Sterile filtered solution containing 10 mM sodium phosphate, pH 7.5
Purity:
≥95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤ 250 pg/mL, determined by the proliferation of 7TD1 cells. ED50 ≤ 25 pg/mL, determined by the proliferation of B9 cells.
Specific Activity:
4.0 x 10e6 U/mg, 7TD1 cell proliferation. 4.0 x 10e7 U/mg, B9 cell proliferation.
Endotoxin Level:
≤1 EU/µg, kinetic LAL
Amino Acid Sequence:
MPVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ DMTTHLILRS FKEFLQSSLR ALRQM
Reconstitution:
Centrifuge vial prior to opening. Add sterile 10 mM HCl to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Upon receipt, store at -20 °C to -80 °C for up to 1 year.
