Recombinant Human IL-6

Molecule:

IL-6

Synonyms:

IL-6, BSF2, HGF, HSF, IFNB2, B-cell differentiation factor

Species:

Human

Cat. No.:

CRI106C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,720.00 / Special Price: $2,100.00 BUY

Description:

Interleukin-6 (IL-6) acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages and plays an essential role in the final differentiation of B-cells into Ig-secreting cells and is involved in lymphocyte and monocyte differentiation. In addition, IL-6 has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. IL-6 is produced at sites of acute and chronic inflammation, where it is secreted and signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal transducing component gp130 (CD130.

Entrez Symbol:

IL6

Uniprot Name:

Interleukin-6

Uniprot ID:

P05231

Data PDF:

CRI106

Source:

E. coli

Molecular Weight:

Monomer, 21 kDa (185 aa)

Physical State:

Lyophilized

Formulation:

Sterile filtered solution containing 10 mM sodium phosphate, pH 7.5

Purity:

≥95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 250 pg/mL, determined by the proliferation of 7TD1 cells. ED50 ≤ 25 pg/mL, determined by the proliferation of B9 cells.

Specific Activity:

4.0 x 10e6 U/mg, 7TD1 cell proliferation. 4.0 x 10e7 U/mg, B9 cell proliferation.

Endotoxin Level:

≤1 EU/µg, kinetic LAL

Amino Acid Sequence:

MPVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ DMTTHLILRS FKEFLQSSLR ALRQM

Reconstitution:

Centrifuge vial prior to opening. Add sterile 10 mM HCl to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Upon receipt, store at -20 °C to -80 °C for up to 1 year.