Synonyms:
Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7
Description:
Recombinant IL-7 is a single polypeptide chain comprised of 153 amino acids.
Background: IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. IL-7 is constitutively produced by stromal cells from the bone marrow and thymus and can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells. IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2.
Uniprot Name:
Interleukin-7
Molecular Weight:
17.5 kDa
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile filtered solution of 10mM acetic acid.
Biological Activity:
The ED50, as determined by the dose-dependent stimulation of the proliferation of mouse 2E8 cells, is less than 1 ng/ml.
Specific Activity:
>1 x 10e6 units/mg
Endotoxin Level:
< 1 EU/μg as determined by Kinetic LAL method.
Amino Acid Sequence:
MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. Allow several minutes for complete reconstitution. DO NOT VORTEX. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Upon receipt, store desiccated at -20°C for up to 1 year. After reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.
