Synonyms:
IL-7, Lymphopoietin, pre-B cell factor
Description:
IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. IL-7 is constitutively produced by stromal cells from the bone marrow and thymus and can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells. IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and mouse IL-7 show species cross-reactivity.
Uniprot Name:
Interleukin-7
Molecular Weight:
Monomer, 17.5 kDa (153 aa)
Physical State:
Lyophilized
Formulation:
Sterile filtered solution of 10mM acetic acid.
Purity:
> 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤ 1 ng/ml, as determined by the dose-dependent stimulation of the proliferation of mouse 2E8 cells.
Specific Activity:
3.5 x 10e6 U/mg
Endotoxin Level:
≤ 1 EU/µg, kinetic LAL
Amino Acid Sequence:
MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. Allow several minutes for complete reconstitution. DO NOT VORTEX. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Store at -20 °C to -80 °C for up to 1 year.
