Recombinant Human IL-7

Molecule:

IL-7

Synonyms:

IL-7, Lymphopoietin, pre-B cell factor

Species:

Human

Cat. No.:

CRI108C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$4,820.00 / Special Price: $3,720.00 BUY

Description:

IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. IL-7 is constitutively produced by stromal cells from the bone marrow and thymus and can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells. IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and mouse IL-7 show species cross-reactivity.

Entrez Symbol:

IL7

Uniprot Name:

Interleukin-7

Uniprot ID:

P13232

Source:

E. coli

Molecular Weight:

Monomer, 17.5 kDa (153 aa)

Physical State:

Lyophilized

Formulation:

Sterile filtered solution of 10mM acetic acid.

Purity:

> 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 1 ng/ml, as determined by the dose-dependent stimulation of the proliferation of mouse 2E8 cells.

Specific Activity:

3.5 x 10e6 U/mg

Endotoxin Level:

≤ 1 EU/µg, kinetic LAL

Amino Acid Sequence:

MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. Allow several minutes for complete reconstitution. DO NOT VORTEX. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store at -20 °C to -80 °C for up to 1 year.