IGF-I

IGF1 , IGF-I , IGFI , IGF-I , IGF1A

Human

CM001 pdf (datasheet)

1.0 mg

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Recombinant human IGF-1 is a single, non-glycosylated polypeptide containing 70 amino acids and 3 intra-molecular disulfide bonds.
Background: Insulin-like growth factor 1 (IGF-I) is a member of the IGF family of mitogenic peptide growth factors, along with insulin and IGF-II. IGF-I stimulates proliferation and survival of various cell types including muscle, bone, and cartilage tissue. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/ autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH). Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR). Proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions, generates mature IGFs.

IGF1

CM001

E. coli

~7.7 kDa

Lyophilized

Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic acid (TFA).

>98% by SDS-PAGE and HPLC

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of mouse FDC-P1 cells, is typically less than 1.0 ng/ml.

> 1 x 10e6 U/mg

<1 EU/μg as determined by kinetic LAL analysis.

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Lyophilized protein is stable at room temperature for shipping. On receipt, store desiccated at -20°C. After reconstitution, stable for one month at 2-8°C. For longer term, store aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.