Recombinant Human Des [1-3] IGF-I, Receptor Grade

Molecule:

IGF-I

Synonyms:

IGF-I, somatomedin C, Mechano growth factor, MGF

Species:

Human

Cat. No.:

DU100 pdf (datasheet)

Quantity/Size:

100 µg

Price:

$290.00 BUY

Description:

Human Des(1-3) Insulin-like Growth Factor-I is a 67 amino acid analog of human IGF-I lacking the N-terminal tripeptide Gly-Pro-Glu. Human Des(1-3) IGF-I is more potent than IGF-I in vitro and in vivo. This increased potency is due to reduced binding of human Des (1-3) IGF-I to most of the IGF binding proteins which normally inhibit the biological actions of IGF-I. Human Des(1-3) IGF-I binds to the Type 1 IGF receptor with similar affinity to wild-type IGF-I.

This is a high quality reagent for use in studies on cell growth, IGF receptors and IGFBPs.

Entrez Symbol:

IGF1

Uniprot ID:

P05019

Data PDF:

DU100

Source:

E. coli

Molecular Weight:

7370±2 daltons

Formulation:

Lyophilized from sterile-filtered 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa).

Purity:

> 95 % by HPLC analysis

Biological Activity:

Stimulation of protein synthesis in rat L6 myoblasts: ED50 < 10 ng/ml

Type 1 IGF receptor binding assay: ED50 < 10 ng/mL

IGF binding protein assay: ED50 > 100 ng/ml

Endotoxin Level:

<0.1 EU/µg

Amino Acid Sequence:

Confirmed 5 residues

Storage/Stability:

Store as supplied for up to 1 year at 2-8°C

Application notes:

Protocol 3001: Iodination of IGF peptides

Protocol 3002: Determination of IGF-I or IGF-II in a range of species by Radioimmunoassay (RIA)