Recombinant Human Insulin-like Growth Factor-II Receptor Grade

Molecule:

IGF-II

Synonyms:

IGF-2, Insulin-like growth factor II, Somatomedin A

Species:

Human

Cat. No.:

FM001 pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,130.00 BUY

Description:

Human Insulin-like Growth Factor-II (IGF-II) is a single chain 67 amino acid polypeptide that shares structural homology with insulin and human IGF-I. IGF-II plays a critical role in embryonic and foetal development via autocrine, paracrine and endocrine mechanisms. It is a mitogen and also has effects on cell migration and differentiation. IGF-II binds to the type I IGF receptor and the IGF-II/cation-independent mannose-6-phosphate receptor. Its actions are probably mediated through inducing signal transduction via one or both of these receptors. IGF-II also binds to each of the six IGF binding proteins (IGFBPs). This binding also regulates the biological function of IGF-II. IGF-II is highly conserved across species which suggests an important role for the peptide.

Human IGF-II is present in high concentrations in normal adult human serum, although the function of this is unknown. IGF-II is also implicated in the pathophysiology of cancer, diabetes and obesity. 

Entrez Symbol:

IGF2

Uniprot Name:

Insulin-like growth factor II

Uniprot ID:

P01344

Data PDF:

FU020

Source:

E. coli

Molecular Weight:

7469 daltons

Physical State:

Lyophilized

Formulation:

0.1 M acetic acid

Purity:

>95% by HPLC

Biological Activity:

Proliferation of Chinese hamster ovary (CHO) cells (ED50 < 150 ng/ml)
IGF type 1 receptor binding assay (ED50 < 20 ng/ml)
IGF type 2 receptor binding assay (ED50 < 70 ng/ml)
IGF binding protein assay (ED50 < 10 ng/ml)

Endotoxin Level:

<0.1 EU/µg.

Amino Acid Sequence:

Confirmed N-terminal 5 residues

Reconstitution:

See Protocol 1000, Handling of IGF-I, IGF-II and IGF analogs.

Storage/Stability:

Store at 2-8°C.

Application notes:

Protocol 3001: Iodination of IGF peptides
Protocol 3002: Determination of IGF-I or IGF-II in a range of species by Radioimmunoassay (RIA)
Protocol 3003 Procedure for Western Ligand blotting using iodinated IGF-I or IGF-II
Protocol 3004 Procedure for Western Ligand blotting using biotinylated IGF-I,  IGF-II