Recombinant Human KDM5D, His tagged

Synonyms:

HY, HYA, SMCY, Histone demethylase JARID1D, Histocompatibility Y antigen

Species:

Human

Cat. No.:

CS414 pdf (datasheet)

Quantity/Size:

25 μg

Price:

$545.00 BUY

Description:

H-Y Antigen/ KDM5D is a protein containing zinc finger domains that specifically demethylates 'Lys-4' of histone H3. The gene is expressed ubiquitously in male mice and humans. A short peptide derived from this protein is a minor histocompatibility antigen which can lead to graft rejection of male donor cells in a female recipient. H-Y antigen appears to be an integral part of the membrane of most male cells and can be detected by cytotoxic T cells and antibodies. In addition, H-Y antibodies detect a soluble form of H-Y that is secreted by the testis and was found to be identical to Müllerian-inhibiting substance (MIS). MIS cross-reacts with H-Y antibodies and identical findings were obtained for soluble H-Y antigen and MIS, i.e., secretion by testicular Sertoli and, to a lesser degree, ovarian cells, binding to a gonad-specific receptor, induction of gonadal sex reversal in vitro and, in cattle, in vivo. H-Y antisera also detect a molecule or molecules associated with the heterogametic sex in non-mammalian vertebrates.

Recombinant Human H-Y Antigen/ KDM5D is a 301 amino acid protein containing a 6x His tag at the C-terminal end.

Entrez Symbol:

KDM5D

Uniprot Name:

Lysine-specific demethylase 5D

Uniprot ID:

Q9BY66

Data PDF:

CS414

Source:

E. coli

Molecular Weight:

33.87 kDa

Tag:

C-terminal 6x His

Formulation:

Lyophilized from 50 mM NaP + 300 mM NaCl, pH 8.0.

Purity:

> 95% by SDS-PAGE, visualized by silver stain

Amino Acid Sequence:

ALLVALQRLP VRLPEGEALQ CLTERAIGWQ DRARKALASE DVTALLRQLA ELRQQLQAKP RPEEASVYTS ATACDPIREG SGNNISKVQG LLENGDSVTS PENMAPGKGS DLELLSSLLP QLTGPVLELP EAIRAPLEEL MMEGDLLEVT LDENHSIWQL LQAGQPPDLD RIRTLLELEK FEHQGSRTRS RALERRRRRQ KVDQGRNVEN LVQQELQSKR ARSSGIMSQV GREEEHYQEK ADRENMFLTP STDHSPFLKG NQNSLQHKDS GSSAACPSLM PLLQLSYSDE QQLLEHHHHH H

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile, distilled water. After reconstitution the protein may be diluted with other aqueous buffers.

Storage/Stability:

Stable at -20°C to -80°C.