Synonyms:
LIF; CDF; DIA; HILDA; MLPLI
Description:
Recombinant human LIF is a non-glycosylated protein containing 181 amino acids.
Background: Leukemia Inhibitory Factor (LIF) is a member of the IL-6 family and a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.
Uniprot Name:
Leukemia inhibitory factor
Molecular Weight:
19.9 kDa
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).
Biological Activity:
Fully biologically active when compared to standard. The specific activity is determined by the ability to induce proliferation of TF-1 cells and is typically less than 0.07 ng/ml.
Specific Activity:
Typically 1 x 107 U/mg
Endotoxin Level:
≤1 EU/µg of protein by kinetic LAL analysis
Amino Acid Sequence:
MSPLPITPVN ATCAIRHPCH NNLMNQIRSQ LAQLNGSANA LFILYYTAQG EPFPNNLDKL CGPNVTDFPP FHANGTEKAK LVELYRIVVY LGTSLGNITR DQKILNPSAL SLHSKLNATA DILRGLLSNV LCRLCSKYHV GHVDVTYGPD TSGKDVFQKK KLGCQLLGKY KQIIAVLAQA F
Reconstitution:
Centrifuge vial prior to opening. Add sterile 10mM acetic acid to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.
