Recombinant Human LR3 IGF-1

Molecule:

IGF-I

Synonyms:

Lr3 IGF-1, LR3 IGF, IGF LR3, LArg3 IGF-1

Species:

Human

Cat. No.:

LRM001 pdf (datasheet)

Quantity/Size:

1 mg

Price:

$250.00 BUY

Description:

IGF-1 belongs to the insulin gene family and is a mitogenic polypeptide growth factor that stimulates the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue.

LR3 IGF-1 is a long-term acting analog of human IGF-1 specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals. It differs from native IGF-1 in that it possesses an arginine instead of a glutamic acid at the third position in its amino acid sequence ("arginine 3"), and also has an additional 13 amino acids at N-terminus (MFPAMPLLSLFVN) ("long"), for a total of 83 amino acids (relative to the 70 of IGF-1).

Entrez Symbol:

IGF1

Data PDF:

LRM001

Source:

E. coli

Molecular Weight:

~9.1 kDa

Formulation:

Lyophilized from sterile-filtered 20 mM phosphate buffer, pH 7.2.

Purity:

>98% by SDS-PAGE analysis, >90% by RP-HPLC

Biological Activity:

ED50 ~0.3-1.5 ng/ml, determined in a serum-free cell proliferation assay using human MCF-7 cells
ED50 <10 ng/ml, determined by the stimulation of protein synthesis in rat L6 myoblasts.

Specific Activity:

> 6.7 × 105 IU/mg, by serum-free cell proliferation assay using human MCF-7 cells
>1.0 x 105 IU/mg, by the stimulation of protein synthesis in rat L6 myoblasts

Endotoxin Level:

<0.01 EU/μg

Amino Acid Sequence:

MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Upon receipt, the lyophilized protein is stable for one year when stored at -20°C to -80° C. When reconstituted under sterile conditions, product is stable for one month at 2-8°C, or for 3 months when stored in working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Application notes:

Mammalian cell culture