Recombinant Human Macrophage Colony Stimulating Factor, Animal Free

Molecule:

CSF1

Synonyms:

M-CSF, CSF-1, MCSF

Species:

Human

Cat. No.:

CRM151C-AF pdf (datasheet)

Quantity/Size:

1 mg

Price:

$4,310.00 / Special Price: $3,320.00 BUY

Description:

Macrophage Colony Stimulating Factor (M-CSF), also called CSF-1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone), exerts paracrine effects on osteoclasts, and can interact with CSF1R. M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer thought to be produced by proteolytic cleavage of membrane-bound precursors.

Manufactured without Animal-derived products, in an Animal Free facility.

Entrez Symbol:

CSF1

Uniprot ID:

P09603

Data PDF:

CRM151-AF

Source:

E. coli

Molecular Weight:

Dimer, 18.5/37.1 kDa (159/318 aa)

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered solution in 10 mM sodium phosphate, 100 mM sodium chloride, pH 8.0.

Purity:

>95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤10 ng/ml, determined by a cell proliferation assay using mouse NFS-60 cells.

Specific Activity:

≥ 1 x 10e5 units/mg, calibrated against recombinant human M-CSF WHO International Standard (NIBSC code: 89/512).

Endotoxin Level:

≤1 EU/µg by kinetic LAL

Amino Acid Sequence:

MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS

Storage/Stability:

Store as supplied at -20 °C to -80 °C for up to one year.