Recombinant Human M-CSF/CSF1 HQ, Endotoxin Free

Molecule:

CSF1

Synonyms:

CSF1, MCSF, CSF-1, M-CSF

Species:

Human

Cat. No.:

CRM146B pdf (datasheet)

Quantity/Size:

50 μg

Price:

$576.00 BUY

Description:

Macrophage colony stimulating factor, colony stimulating factor 1, CSF-1, M-CSF is a hematopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological activity on mouse and rat cells and although mouse M-CSF is active in rat cells it is inactive in human cells.

Uniprot Name:

Macrophage colony-stimulating factor 1

Uniprot ID:

P09603

Data PDF:

CRM146

Source:

Barley

Molecular Weight:

Recombinant human M-CSF contains 159 aa with a 16 aa His tag for a total length of 175 aa, a predicted molecular mass of 20.7 kDa, and an apparent molecular mass of 24 -26 kDa by SDS-PAGE.

Formulation:

Lyophilized from PBS, pH 7.2, sterile filtered through a 0.2 µm filter.

Purity:

> 95% by SDS-PAGE gel analysis.

Biological Activity:

ED50 <2 ng/ml. Bioactivity is assayed by measuring the dose-dependent effect of human M-CSF on proliferation of M-NFS-60 cells.

Specific Activity:

> 5 x 10E5 U/mg

Endotoxin Level:

Endotoxin level is less than 0.005 ng per μg (0.05 EU/μg) as measured by turbidimetric kinetic assay.

Reconstitution:

Centrifuge vial prior to opening. Reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 µg/ml. The solution can be further diluted into aliquots in aqueous buffers. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Storage/Stability:

The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.