Recombinant Human MAPK12/p38 gamma, Active

Species:

Human

Cat. No.:

CRP103B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$495.00 BUY

Description:

MAPK12 is a member of the p38 MAPK family which is activated in response to stress. MAPK12 gene was mapped to 22q13.3 and functions as a signal transducer during differentiation of myoblasts to myotubes. Enforced localization of MAPK12 in the nucleus or cytoplasm markedly attenuates the ability of the kinase to induce cell cycle arrest in fibroblasts. MAPK12 increases basal glucose uptake and decreases DNP- and contraction-stimulated glucose uptake, partially by affecting levels of glucose transporter expression in skeletal muscle.

Concentration:

0.1 mg/ml

Entrez Symbol:

MAPK12

Uniprot Name:

Mitogen-activated protein kinase 12

Uniprot ID:

P53778

Data PDF:

CRP103

Source:

Insect cells

Molecular Weight:

approximately 71 kDa

Tag:

N-terminal GST

Formulation:

50 mM Tris-HCl, 150 mM NaCl, 10 mM glutathoine, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% Glycerol, pH 7.5

Purity:

> 90% by SDS-PAGE (Coomassie)

Specific Activity:

> 200 nmol/min/mg:, by Kinase Specific Activity: nmol phosphate incorporated into the PKB-sub peptide (CKRPRAASFAE) per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Storage/Stability:

Store in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.