Recombinant Human Matrix Metalloproteinase 3

Cat. No.:

CRM012B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$300.00 BUY

Description:

Recombinant Human MMP-3 produced in HEK293 cells is the proform of the Human MMP3 [Tyr18-Cys477 (Lys45Glu)] and fused with a C-terminal polyhistidine tag. MMP-3 enzyme is also known as Stromelysin-1or as Transin-1 which hydrolyzes natural collagen at physiological pH and temperature. It dissolves the intervertebral nucleus pulposus and annulus fibrosus of Herniated Lumbar Intervertebral Disk . MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and superactivates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. MMP-3 was found to be capable of activating the precursor of IL1-beta.

Entrez Symbol:

MMP2

Uniprot Name:

Stromelysin-1

Uniprot ID:

P08254

Data PDF:

CRM012

Source:

HEK293 cells

Molecular Weight:

52 kDa

Physical State:

Liquid

Formulation:

Sterile-filtered 20 mM Tris, 150 mM NaCl and 0.05% Brij35, pH 7.5

Purity:

> 95% as determined by SDS-PAGE.

Biological Activity:

The activity was measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is > 150 pmoles/min/µg.
Recombinant Human MMP-3 protein pro form needs to be activated with Chymotrypsin.
Activation Protocol:
1. Dilute MMP3 to 20µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.
2. Activate MMP3 by adding Chymotrypsin(Sigma, Catalog#C­3142,1mg/ml stock in 1mM HCl) to a final concentration of 5ug/ml.
3. Incubate at 37°C for 30 minutes.
4. Stop activation with 2mM PMSF. Pre-warm the PMSF to 37°C prior to adding to sample.

Specific Activity:

> 150 pmoles/min/µg

Storage/Stability:

Store at -20°C to -80°C.