Synonyms:
Macrophage migration inhibitory factor, Glycosylation-inhibiting factor, GIF
Description:
Macrophage migration inhibitory factor (MIF) is a pro-inflammatory lymphokine that functions during cell-mediated immmunity. MIF promotes fibroblast migration by inducing interleukin 1 (IL-1), interleukin 8 (IL-8), and matrix metalloproteinase (MMP) expression. In interferon-gamma-activated macrophages, MIF stimulates nitric oxide (NO) production and tumor necrosis factor-alpha (TNF-α) secretion.
Uniprot Name:
Macrophage migration inhibitory factor
Molecular Weight:
Monomer, 12.5 kDa (115 aa)
Formulation:
Lyophilized from a sterile-filtered solution containing 10 mM sodium phosphate, pH 7.5
Purity:
≥95% by reducing and non-reducing SDS-PAGE
Biological Activity:
This product demonstrates dose-dependent induction of IL-8 in human PBMCs. No acceptance criteria established.
Endotoxin Level:
≤1 EU/µg by kinetic LAL analysis
Amino Acid Sequence:
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year.