Human Recombinant Afadin (aa 1-141) Ras-binding Domain, GST-tag

Synonyms:

Human Recombinant Afadin (aa 1-141) Ras-binding Domain, GST-tag

Species:

Human

Cat. No.:

CSI13809 pdf (datasheet)

Quantity/Size:

50 μg

Price:

$490.00 BUY

Description:

The N-terminus of Af6 was identified as the Ras-binding site. Additionally to the Rasbinding Af6 also binds Rap. ZO-1, a protein involved in the formation of tight junctions, also binds to Af6 close to the amino terminus thereby competing with Ras binding. This data suggests a participation of Af6 in the regulation of cell-cell contacts via a Rasmodulated interaction with ZO-1. In contrast to the well characterized effectors Raf, RalGEF, and PI(3)kinase, Af6 is a protein with no enzymatic function. It seems to serve as a scaffolding component for protein complexes. This would represent a new type of Ras effector because Ras binding in this case does not lead to the activation of the enzymatic activity of the effector.

The GST-Tag facilitates the protein‘s application in typical GST pull down assays.

Uniprot Name:

Afadin

Uniprot ID:

P55196

Data PDF:

CSI13809

Source:

E. coli

Formulation:

25 mM HEPES, 50 mM NaCl, 1 mM EDTA, 50% glycerol, pH 7.5

Purity:

> 95% by SDS-PAGE

Storage/Stability:

Store at -80°C for up to 1 year. Upon initial thaw, prepare aliquots and store at -80°C. Avoid freeze-thaw cycles.