Recombinant Human Multimeric CD40L

Synonyms:

CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L

Species:

Human

Cat. No.:

CRH024 pdf (datasheet)

Quantity/Size:

10 μg

Price:

$431.00 BUY

Description:

Recombinant human multimeric CD40L is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of mouse ACRP30 (adipogen, Adipoq). This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to using a combination of CD40L plus an enhancer to increase efficacy. Multimeric CD40L has been shown to suppress alum-induced IL-1β release and caspase-1 activation in a CD40-, dose-, and time-dependent manner without affecting BMDM viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.

Concentration:

0.1 mg/ml after reconstitution

Uniprot ID:

P29965 CD40L/Q60994 ACRP30 mouse precursor

Data PDF:

CRH024

Source:

CHO cells

Formulation:

Lyophilized solution containing PBS.

Purity:

≥95% (SDS-PAGE)

Biological Activity:

Induces B cells activation as demonstrated by the dose-dependent upregulation of CD86, ED50 <1 ng/ml.

Endotoxin Level:

<0.01 EU/μg purified protein as determined by LAL test (Lonza).

Amino Acid Sequence:

Human CD40L aa 116-261 is fused at the N-terminus to mouse collagen domain of ACRP30 (aa18-111) and a FLAG tag.

Reconstitution:

Reconstitute with 100μl sterile water.

Storage/Stability:

Store at -20°C to -80°C . After reconstitution, prepare aliquots and store at -20°C to -80° C. PBS containing at least 0.1% BSA or HSA should be used for further dilution. Avoid repeated freeze-thaw cycles.