Recombinant Human Multimeric TNF-alpha

Molecule:

TNF-alpha

Synonyms:

Multimeric TNF-α, ACRP30headless:TNF-α, ACRP30headless:TNFSF2, ACRP30headless:Tumor Necrosis Factor-α

Species:

Human

Cat. No.:

CRH022 pdf (datasheet)

Quantity/Size:

10 μg

Price:

$417.00 BUY

Description:

The extracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG-tag.

Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-α) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. Multimeric TNF-α is a high activity construct in which two trimeric TNF-α ligands are artificially linked via the collagen domain of mouse ACRP30 (adiponectin, Adipoq). The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-α, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-α. Since Multimeric TNF-α mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-α protein that can activate the TNF-R2. For TNF-R1 activation, either "regular" recombinant TNF-α or Multimeric TNF-α can be used.

Concentration:

0.1 mg/ml after reconstitution

Uniprot Name:

Tumor necrosis factor / Adiponectin

Uniprot ID:

P01375 / Q60994

Data PDF:

CRH022

Source:

HEK293 cells

Molecular Weight:

~34 kDa by SDS-PAGE

Formulation:

Lyophilized solution containing PBS

Purity:

≥95% (SDS-PAGE)

Biological Activity:

Activates human and mouse TNF-R1 and TNF-R2. Induces cell death of WEHI 164 cells at a concentration range of 0.01-10 ng/ml, ED50 = 0.05 ng/ml or 2 x 10e7 Units/mg.

Specific Activity:

2 x 107 Units/mg

Endotoxin Level:

<0.02 EU/μg purified protein as determined by LAL test (Lonza).

Amino Acid Sequence:

FLAG tag + mouse collagen domain of ACRP30 (aa 18-111) + Human TNF-alpha (aa 85 -233)

Reconstitution:

Reconstitute with 100μl sterile water.

Storage/Stability:

Store at -20°C to -80°C for at least 6 months. After reconstitution, prepare aliquots and store -20°C to -80°C for up to 3 months. PBS containing at least 0.1% BSA or HSA should be used for further dilution. Avoid repeated freeze-thaw cycles.