Recombinant Human Myostatin

Synonyms:

Growth/differentiation factor 8, GDF-8

Species:

Human

Cat. No.:

CRMY00B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$185.00 BUY

Description:

Myostatin is a member of the TGF-beta superfamily that is essential for proper regulation of skeletal muscle mass in a number of species ranging from fish to humans. Myostatin is a secreted protein that negatively regulates skeletal muscle mass determining both muscle fiber number and size. Inhibition of myostatin can increase muscle mass in a number of animal models of human disease, including muscular dystrophy. Recombinant human Myostatin is a non-glycosylated homodimer, containing two 109 amino acid chains.

Entrez Symbol:

MSTN

Uniprot Name:

Growth/differentiation factor 8

Uniprot ID:

O14793 (Asp267-Ser375)

Source:

E. coli

Molecular Weight:

12.4/24.8 kDa (109/218 aa)

Formulation:

Lyophilized from a sterile-filtered solution containing 0.1% Trifluoroacetic (TFA)

Purity:

≥ 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

< 50 ng/ml determined by cytotoxicity of MPC-11 cells

Specific Activity:

≥ 2.0 x 104 units/mg

Endotoxin Level:

≤ 1 EU/μg, by kinetic LAL analysis

Amino Acid Sequence:

DFGLDCDEHS TESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGECEFV FLQKYPHTHL VHQANPRGSA GPCCTPTKMS PINMLYFNGK EQIIYGKIPA MVVDRCGCS

Reconstitution:

Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 20 mM HCl at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Storage/Stability:

Upon receipt, store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8o C. For long term storage, reconstitute in working aliquots in 0.1% BSA solution and store at -80o C. Avoid repeated freeze-thaw cycles.