Recombinant Human PDGF-AB Animal Free

Synonyms:

Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF

Species:

Human

Cat. No.:

CRP301C-AF pdf (datasheet)

Quantity/Size:

1 mg

Price:

$4,560.00 / Special Price: $2,980.00 BUY

Description:

Platelet-Derived Growth Factor (PDGF) plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum.The synthesis of PDGF can be induced by IL-1, IL-6, TNF-alpha, TGF-beta and EGF. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β.

Manufactured without Animal-derived products, in an Animal Free facility.

Uniprot ID:

P04085, P01127

Data PDF:

CRP301-AF

Source:

E. coli

Molecular Weight:

Dimer, Alpha:14.4 kDa, Beta: 12.4 kDa, Total 26.8 kDa (Alpha: 126, Beta: 110, Total: 236 aa)

Physical State:

Lyophilized

Formulation:

Sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Purity:

≥ 95%, determined by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 2 ng/ml, determined by the dose-dependent proliferation of mouse 3T3 indicator cells.

Specific Activity:

> 5.0 x 10e4 U/mg

Endotoxin Level:

≤ 1 EU/μg protein, by kinetic LAL analysis

Amino Acid Sequence:

Alpha chain MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT Beta chain MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIF

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year.