Recombinant Human PDGF-BB

Molecule:

PDGFB

Synonyms:

PDGF-2, PDGF2, SIS, SSV, IBGC5, c-sis, GDGF, ODGF

Species:

Human

Cat. No.:

CRP302C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,520.00 / Special Price: $2,740.00 BUY

Description:

Platelet-Derived Growth Factor (PDGF) is a member of the protein family which includes vascular endothelial growth factors (VEGF). PDGF is proteolytically processed from a pre/pro-protein to generate PDGF subunit B, which can homodimerize, or can heterodimerize with the related PDGF subunit A. These proteins bind and activate PDGF receptor tyrosine kinases. PDGF is a mitogenic peptide growth hormone carried in the alpha-granules of platelets, released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF.

Entrez Symbol:

PDGFB

Uniprot Name:

Platelet-derived growth factor subunit B

Uniprot ID:

Platelet-derived growth factor subunit B

Data PDF:

CRP302

Source:

E. coli

Molecular Weight:

Dimer, 12.4/24.9 kDa (110/220 aa)

Formulation:

Lyophilized from sterile filtered 10 mM sodium phosphate, pH 7.5

Purity:

≥95.0% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 20 ng/ml, determined by the dose-dependant proliferation of 3T3 cells

Specific Activity:

≥ 5.0 x 104 IU/mg, calibrated against the International Standard, NIBSC code: 94/728

Endotoxin Level:

≤ 1 EU/µg by kinetic LAL

Amino Acid Sequence:

MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1 mg/mL by gently pipetting and washing down the sides of the vial to ensure full recovery of the protein. Allow several minutes to ensure full solubilization. DO NOT VORTEX.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.