Recombinant Human Persephin

Synonyms:

PSP, PSPN

Species:

Human

Cat. No.:

CRP400B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$520.00 BUY

Description:

Persephin is a disulfide-linked homodimer neurotrophic factor structurally related to GDNF, Artemin, and Neurturin. Each chain contains seven conserved cysteine residues, one of which (Cys 63) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system, composed of RET and one of four GFR α (α1-α4) receptors. The GFRα4 was first identified in chicken and was later shown to be the preferential binding subunit for Persephin. Persephin promotes the survival of ventral midbrain dompaminergic neurons and motor neurons after sciatic nerve oxotomy, and like GNDF, promotes ureteric bud branching. However, in contrast to GDNF and Neurturin, Persephin does not support survival of peripheral neurons.

Entrez Symbol:

PSPN

Uniprot Name:

Persephin

Uniprot ID:

O60542

Data PDF:

CRP400

Source:

E. coli

Molecular Weight:

10.4 kDa (194 aa) monomer

Physical State:

Lyophilized

Formulation:

Lyophilized from sterile-filtered 0.3X PBS, pH 7.2.

Purity:

>90% by SDS-PAGE and HPLC analyses

Biological Activity:

Human Persephin induces RET phosphorylation using a concentration range of 0.1-1.0 ng/ml. Human Persephin binds to mammalian GFRa4 with the Kd of 100 pM. Other members of the GDNF family (Artemin, GDNF and Neurturin) do not bind to mammalian GFRa4.

Endotoxin Level:

<1 EU/µg

Storage/Stability:

Stable for 1 year at -20°C to -80°C.