Recombinant Human PLA2G1B/Phospholipase A2-IB His

Synonyms:

Group IB phospholipase A2, Phosphatidylcholine 2-acylhydrolas 1B

Cat. No.:

CSI10728 pdf (datasheet)

Quantity/Size:

10 μg

Price:

$300.00 BUY

Description:

Group IB secretory phospholipase A2 (sPLA2-IB) mediates cell proliferation, cell migration, hormone release and eicosanoid production via its receptor in peripheral tissues. In the CNS, high-affinity binding sites of sPLA2-IB have been documented. sPLA2-IB induced neuronal cell death in a concentrationdependent manner depending on PGD2 metabolites, especially Delta12-PGJ2 that might mediate sPLA2-IB-induced apoptosis. The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of lowmolecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.

Uniprot ID:

P04054

Data PDF:

CSI10727

Source:

E. coli

Molecular Weight:

16 kDa (126 aa)

Formulation:

Lyophilized from filtered (0.4 µm) 0.05 M acetate buffer, pH 4

Purity:

> 95% as determined by SDS-PAGE.

Amino Acid Sequence:

MRGSHHHHHH GMASHMAVWQ FRKMIKCVIP GSDPFLEYNN YGCYCGLGGS GTPVDELDKC CQTHDNCYDQ AKKLDSCKFL LDNPYTHTYS YSCSGSAITC SSKNKECEAF ICNCDRNAAI CFSKAPYNKA HKNLDTKKYC QS

Reconstitution:

Add 0.1 M Acetate buffer pH 4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 μg/ml. In higher concentrations the solubility of this antigen is limited.

Storage/Stability:

Store lyophilized protein at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare aliquots and store at -20°C to -80°C.

Application notes:

Product is not sterile. Please filter the product by an appropriate sterile filter before using it in the cell culture.