Recombinant Human Placenta Growth Factor 3, Animal Free

Molecule:

PGF

Synonyms:

PIGF

Species:

Human

Cat. No.:

CRH067C-AF pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,990.00 / Special Price: $2,440.00 BUY

Description:

PlGF, a member of the VEGF family, was originally discovered in the placenta, where it was proposed to regulate vascular development and trophoblast growth and differentiation. Unlike VEGF, which is required for angiogenesis and endothelial cell maintenance and binds both Flt-1/sFlt-1 and Flk-1, PlGF is redundant for vascular development and selectively binds Flt-1/sFlt-1. PlGF stimulates endothelial cell growth, migration, and survival and plays a central role in pathologic angiogenesis, including in cancer and tissue ischemia. PlGF concentrations increase throughout pregnancy, peaking during the third trimester, and falling thereafter, probably as a consequence of placental maturation. Despite interest in the utility of PlGF for clinical diagnostics, the role of PlGF in preeclampsia and in placental development is not well understood.

Manufactured without Animal-derived products, in an Animal Free facility.

Entrez Symbol:

PGF

Uniprot ID:

P49763

Data PDF:

CRH067-AF

Source:

E. coli

Molecular Weight:

22.9/45.8 kDa Dimer, (204/408 aa)

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

≥95% by reducing and non-reducing SDS-PAGE.

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MLPAVPPQQW ALSAGNGSSE VEVVPFQEVW GRSYCRALER LVDVVSEYPS EVEHMFSPSC VSLLRCTGCC GDENLHCVPV ETANVTMQLL KIRSGDRPSY VELTFSQHVR CECRHSPGRQ SPDMPGDFRA DAPSFLPPRR SLPMLFRMEW GCALTGSQSA VWPSSPVPEE IPRMHPGRNG KKQQRKPLRE KMKPERCGDA VPRR

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.

Application notes:

Manufactured without Animal-derived products, in an Animal Free facility.