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Recombinant Human Plasminogen Angiostatin Kringles 1-3

Molecule:

PLG

Species:

Human

Cat. No.:

CRA007B pdf (datasheet)

Quantity/Size:

50 μg

Price:

$275.00 BUY

Description:

Angiostatin is proteolytically processed from plasminogen, a secreted blood zymogen. Angiostatin reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth.

Recombinant Human Angiostatin, Kringle domains 1-3 (Ang K1-3) is a proteolytic fragment of Plasminogen (PLG) containing the first three of Angiostatin's four Kringle domains. Ang K1-3 is more active than Kringles 1-4. Recombinant Human Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids corresponding to residues 97-356.

Entrez Symbol:

PLG

Data PDF:

CRA007

Source:

E. coli

Molecular Weight:

29.7 kDa

Formulation:

Lyophilized from sterile filtered 20 mM Sodium Acetate pH 5.5 + 4% Mannitol

Purity:

>95% by SDS-PAGE and HPLC

Biological Activity:

Fully biologically active compared to standard. The activity is determined by an anti-proliferation and anti-migration assay using endothelial cells in vitro and anti-angiogenesis in vivo.

Specific Activity:

5.5 x 105 Units/mg

Endotoxin Level:

<1 EU/µg of protein

Amino Acid Sequence:

VYLSECKTGN GKNYRGTMSK TKNGITCQKW SSTSPHRPRF SPATHPSEGL EENYCRNPDN DPQGPWCYTT DPEKRYDYCD ILECEEECMH CSGENYDGKI SKTMSGLECQ AWDSQSPHAH GYIPSKFPNK NLKKNYCRNP DRELRPWCFT TDPNKRWELC DIPRCTTPPP SSGPTYQCLK GTGENYRGNV AVTVSGHTCQ HWSAQTPHTH NRTPENFPCK NLDENYCRNP DG

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-8°C, but should be kept desiccated at -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. Store in working aliquots below -20°C. Avoid repeated freeze-thaw cycles.