Recombinant Human proIGF2 (aa 1-156) Receptor Grade

Molecule:

IGF-II

Synonyms:

Pro-Insulin-like growth factor II, pro-IGF-2, Somatomedin-A

Species:

Human

Cat. No.:

AHU020 pdf (datasheet)

Quantity/Size:

20 µg

Price:

$290.00 BUY

Description:

Recombinant Human Insulin-like Growth Factor-II (Human IGF-II) is synthesized as a 156 amino acid precursor protein, known as proIGF-II. This protein includes an 87 amino acid C-terminal region known as the E-domain. A proteolysis step releases the mature 67 amino acid IGF-II polypeptide. The proIGF-II proteins make up 10-20% of circulating IGFII. Pro-IGF-II proteins are secreted by some tumor cell lines and levels are elevated in non-islet cell tumor hypoglycemia.

Entrez Symbol:

IGF2

Uniprot ID:

P01344

Data PDF:

AHU020

Source:

E. coli

Molecular Weight:

Three major isoforms of proIGF-II at 16.1, 17.0 and 17.6 kDa, full length 156 amino acid protein and two smaller species, C-terminal truncations of approximately 5 and 15 residues which result from the purification process.

Formulation:

Lyophilized from 0.1 M acetic acid. Sealed under dry nitrogen at a slight vacuum (-25 kPa).

Purity:

> 85 % by HPLC analysis

Biological Activity:

Stimulation of protein synthesis in rat L6 myoblasts: ED50 < 220 ng/ml

Endotoxin Level:

< 0.1 EU/µg

Amino Acid Sequence:

5 residues confirmed

Storage/Stability:

Stored as supplied for up to 1 year at 2-8°C.

Application notes:

Protocol 3003 Procedure for Western Ligand blotting using iodinated IGF-I or IGF-II

Protocol 3004 Procedure for Western Ligand blotting using biotinylated IGF-I or IGF-II