Recombinant Human PUMA (aa 76-170) His/StrepII Tag

Synonyms:

Bcl-2-binding component 3, isoforms 1/2, JFY-1, p53 up-regulated modulator of apoptosis

Species:

Human

Cat. No.:

CRP170 pdf (datasheet)

Quantity/Size:

50 μg

Price:

$430.00 BUY

Description:

The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53- induced apoptosis.

Concentration:

1.0 mg/ml

Uniprot Name:

Bcl-2-binding component 3, isoforms 1/2

Uniprot ID:

Q9BXH1

Data PDF:

CRP170

Source:

E. coli

Molecular Weight:

15 kDa, predicted

Formulation:

1 x PBS containing 0.1% SDS and 0.02% Sodium Azide.

Purity:

~95%

Storage/Stability:

Store at -80°C. Upon thawing, briefly centrifuge the vial to consolidate product at the bottom of vial. Prepare aliquots > 20 µl, store at -80°C. Avoid repeated freezing and thawing cycles.

Application:

ELISA, MS, WB