Recombinant Human R-Spondin 1

Synonyms:

Roof plate-specific spondin-1, hRspo1, RSPO-1

Species:

Human

Cat. No.:

CRH332C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,020.00 / Special Price: $2,460.00 BUY

Description:

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. RSPO-1 is expressed in many tissues and regulates female sex determination, XX gonad development, oocyte differentiation, and hematopoietic stem cell specification. Recombinant RSPO-1 is a critical protein used to establish 3D intestinal organoid cultures.

Entrez Symbol:

RSPO1

Uniprot ID:

Q2MKA7

Source:

E. coli

Molecular Weight:

Monomer, 25.6 kDa (with 233 amino acids)

Physical State:

Lyophilized

Formulation:

Sterile (0.2 micron) filtered aqueous solution containing phosphate buffered saline (PBS).

Purity:

> 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 50 ng/ml, determined by Luciferase induction in HEK-293 STF cells in the presence of Murine Wnt-3a.

Specific Activity:

> 2.0 x 10e4 U/mg

Endotoxin Level:

≤ 1 EU/µg by kinetic LAL

Amino Acid Sequence:

RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year.