Recombinant Human Serpin F1/PEDF

Molecule:

SERPINF1

Synonyms:

Cell proliferation-inducing gene 35 protein, EPC-1, PEDF, Serpin F1

Species:

Human

Cat. No.:

CRP276B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$275.00 BUY

Description:

Pigment Epithelium-derived Factor (PEDF) is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is a 50 kDa glycoprotein produced and secreted in many tissues throughout the body. A major component of the antiangiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. In addition, PEDF is able to inhibit the activity of angiogenic factors such as VEGF and FGF-2. The neuroprotective effects of PEDF are achieved through suppression of neuronal apoptosis induced by peroxide, glutamate, or other neurotoxins. The recent identification of a lipaselinked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. To date, PEDF-R is the only signaling receptor known to be used by a serpin family member. The unique range of PEDF activities implicate it as a potential therapeutic agent for the treatment of vasculature related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF also has the potential to be useful in the treatment of various angiogenesis-related diseases including a number of cancers.

Entrez Symbol:

SERPINF1

Uniprot Name:

Pigment epithelium-derived factor

Uniprot ID:

P36955

Data PDF:

CRP276

Source:

E. coli

Molecular Weight:

~ 44.4 kDa (399 aa) monomer

Formulation:

Lyophilized from sterile-filtered 20 mM PBS, pH7.4

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

ED50 < 2 ng/ml, determined by its ability to enhance the adhesion of human Saos2 cells to bovine Collagen I coated plate.

Specific Activity:

5.0 × 105 IU/mg

Endotoxin Level:

< 1 EU/μg of rHuPEDF as determined by LAL method

Amino Acid Sequence:

QNPASPPEEG SPDPDSTGAL VEEEDPFFKV PVNKLAAAVS NFGYDLYRVR SSTSPTTNVL LSPLSVATAL SALSLGAEQR TESIIHRALY YDLISSPDIH GTYKELLDTV TAPQKNLKSA SRIVFEKKLR IKSSFVAPLE KSYGTRPRVL TGNPRLDLQE INNWVQAQMK GKLARSTKEI PDEISILLLG VAHFKGQWVT KFDSRKTSLE DFYLDEERTV RVPMMSDPKA VLRYGLDSDL SCKIAQLPLT GSMSIIFFLP LKVTQNLTLI EESLTSEFIH DIDRELKTVQ AVLTVPKLKL SYEGEVTKSL QEMKLQSLFD SPDFSKITGK PIKLTQVEHR AGFEWNEDGA GTTPSPGLQP AHLTFPLDYH LNQPFIFVLR DTDTGALLFI GKILDPRGP

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-8°C, but recommended to store at -20°C to -80°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, store in working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.