Recombinant Human/Mouse TGF-beta 3

Synonyms:

TGF-beta 3, ARVD, ARVD1

Species:

Mouse | Human

Cat. No.:

CRH333C pdf (datasheet)

Quantity/Size:

1 mg

Price:

$4,820.00 / Special Price: $3,940.00 BUY

Description:

The Transforming Growth Factors (TGFs) are multifunctional peptides that regulate growth and differentiation in a variety of cells. Recent data suggests that individual TGFbeta isoforms (TGF-beta1, -beta2 and –beta3) have overlapping, yet distinct biological actions and target cell specificities, both in developing and adult tissues. TGF-beta3 is a new isoform that is presumed to play an important role in wound repair and scarring. TGF-beta3 is also thought to be involved in osteoblast proliferation, chemotaxis, and collagen synthesis. Human and mouse TGF-β3 have 100% sequence identity.

Entrez Symbol:

TGFB3

Uniprot ID:

P10600 human proprotein/P17125 mouse proprotein

Data PDF:

CRH333

Source:

E. coli

Molecular Weight:

12.9/25.7 kDa (113/226 aa), dimer

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Purity:

≥ 95% by reducing and nonreducing SDS PAGE

Biological Activity:

ED50 ≤1 ng/mL, determined by the dose-dependent inhibition of IL-4-induced proliferation from mouse HT-2 cells.

Specific Activity:

≥ 1 x 10e6 U/mg

Endotoxin Level:

≤ 1 EU/μg by kinetic LAL analysis

Amino Acid Sequence:

MALDTNYCFR NLEENCCVRP LYIDFRQDLG WKWVHEPKGY YANFCSGPCP YLRSADTTHS TVLGLYNTLN PEASASPCCV PQDLEPLTIL YYVGRTPKVE QLSNMVVKSC KCS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipet the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.