Recombinant Human TNF-alpha

Molecule:

TNF-alpha

Synonyms:

TNF-alpha, TNFA, TNFSF2, DIF

Species:

Human

Cat. No.:

CRT100A pdf (datasheet)

Quantity/Size:

10 μg

Price:

$85.00 BUY

Description:

Recombinant Human TNF-alpha is a single, non-glycosylated polypeptide chain containing 158 amino acids. Background: Tumor necrosis factor alpha (TNF-alpha) is a multifunctional proinflammatory cytokine that belongs to the TNF superfamily. TNF-alpha is secreted by macrophages, monocytes, neutrophils, T cells, NK-cells following their stimulation by bacterial LPS. TNF-alpha activates signals through two receptors, TNFR1, which is expressed on most cell types, and TNFR2, which is expressed mainly on immune cells. TNF-alpha can have many functions including stimulation of phagocytosis in macrophages, chemoattraction of neutrophils, increase in insulin resistance, and inducing fever, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

Entrez Symbol:

TNF

Uniprot Name:

Tumor necrosis factor

Uniprot ID:

P01375

Data PDF:

CRT100

Source:

E. coli

Molecular Weight:

Monomer, 17.5 kDa (158 aa)

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered solution in 10 mM sodium phosphate, pH 7.5.

Purity:

≥95% by reducing and nonreducing SDS-PAGE

Biological Activity:

The ED50, as determined by cytolysis of mouse L929 cells in the presence of Actinomycin D, is ≤2 ng/mL.

Specific Activity:

≥5 x 10e5 U/mg

Endotoxin Level:

≤1 EU/µg

Amino Acid Sequence:

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Store at -20 °C